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Microbially induced calcite precipitation (MICP) and Enzyme Induced Calcite precipitation (EICP) are sustainable soil improvement techniques. Sporosarcina pasteurii is a non-pathogenic bacteria widely used in lab-scale and field-scale studies of augmented MICP. Media optimization and the analysis of the growth kinetics of S. pasteurii are vital for the application of MICP process. The influence of pH, temperature, and urea concentration on the growth kinetics of S. pasteurii is already established from the previous studies. However, choosing an effective medium for bacterial growth can also significantly influence the growth kinetics and urease activity of S. pasteurii which is missing in most literatures. Keeping this in view, in the current study, the growth kinetics and urease activity of S. pasteurii (NCIM 2477, equivalent to ATCC 11,859) in four different media were analyzed. The best medium was then chosen, and a biomineralization test was performed to evaluate the precipitation efficiency and polymorph type. Further, at similar urease enzyme concentration, the EICP biomineralization test was conducted to analyze the precipitation efficiency and crystal morphology. Thus, the paper compares the precipitation efficiency and crystal morphology in MICP and EICP methods at similar concentrations. Such a study is pivotal to increase the efficiency of MICP and EICP to take these processes to large-scale applications.

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Optimization of Medium for S. pasteurii Bacterium and Performance Comparison with Plant Urease for Geotechnical Applications

  • 会议论文

  • 发表日期：

    2025年

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  • Dhanasree Suresh,
  • Preity Yadav,
  • Kala Venkata Uday

摘要

Microbially induced calcite precipitation (MICP) and Enzyme Induced Calcite precipitation (EICP) are sustainable soil improvement techniques. Sporosarcina pasteurii is a non-pathogenic bacteria widely used in lab-scale and field-scale studies of augmented MICP. Media optimization and the analysis of the growth kinetics of S. pasteurii are vital for the application of MICP process. The influence of pH, temperature, and urea concentration on the growth kinetics of S. pasteurii is already established from the previous studies. However, choosing an effective medium for bacterial growth can also significantly influence the growth kinetics and urease activity of S. pasteurii which is missing in most literatures. Keeping this in view, in the current study, the growth kinetics and urease activity of S. pasteurii (NCIM 2477, equivalent to ATCC 11,859) in four different media were analyzed. The best medium was then chosen, and a biomineralization test was performed to evaluate the precipitation efficiency and polymorph type. Further, at similar urease enzyme concentration, the EICP biomineralization test was conducted to analyze the precipitation efficiency and crystal morphology. Thus, the paper compares the precipitation efficiency and crystal morphology in MICP and EICP methods at similar concentrations. Such a study is pivotal to increase the efficiency of MICP and EICP to take these processes to large-scale applications.