This study takes higher education in Dongguan area as the research object to explore whether organizational commitment and organizational culture affect employees’ job satisfaction and conducts a questionnaire survey with a convenience sampling method for faculty and staff of higher education in Dongguan area. The methods used in this study are narrative statistical analysis, reliability analysis, t-test, one-way variance analysis, and regression analysis. The results of the study show that in the analysis of organizational commitment differences, younger or less senior staff are weaker than senior staff in terms of linking with the organization; staff with a monthly income of more than 60,000 yuan come to identify more strongly with the organizational commitment. In the analysis of organizational culture differences, the time experienced by older and more senior staff in the organization will make them different from younger and less senior staff; the monthly income can reflect the organizational culture and the time experienced by staff in the organization and the degree of identification with the organizational culture. In the difference analysis of job satisfaction, older teaching staff have longer seniority and should receive higher compensation than younger teaching staff, so their job satisfaction is relatively higher; when the income is higher, it means that their rank and seniority are better than other teaching staff, and the higher compensation they receive, the higher their job satisfaction is. The regression analyses show significant positive correlations and are the same as the hypotheses established in the relevant literature.