Enhancing combustion efficiency and minimizing detrimental emissions has been a prominent area of research in the field of engines and its associated fields. In addition to the rapid expansion of the energy industry, the need for renewable energy has been driven by the demand for fuel in household and commercial settings, as well as strict regulations on the emission of greenhouse gases. Recent research is focused on biofuels, which are considered to be an environmentally friendly and sustainable alternative to conventional fossil fuels. Biofuels have become an essential component of contemporary society’s daily existence. Bioethanol and fatty acid methyl esters (FAME) are commonly used in the manufacturing of both gasoline and diesel fuels. Transesterification is a commercially employed technique for producing FAME, a common form of fuel. However, it is associated with significant capital expenses and other complexities. Hydrotreated vegetable oil (HVO), often known as green diesel, is a viable and superior substitute for transesterification in the production of biodiesel. HVO is created by a hydrotreatment method. This fuel has exceptional oxidation stability for long-term storage, a reduced temperature at which it loses its capacity to pass through filters during winter, a lower boiling point to enhance its ability to start in cold conditions, and various other benefits. The density, viscosity, plugging point, and flash point of a gasoline mix derived from HVO have been evaluated to verify its compliance with a fuel standard. These measurements demonstrate that the HVO fuel can be used in engines without any modifications.