Bio-cementation techniques are considered to be a potential solution for improvement of the engineering characteristics of poorly graded loose sand deposits in compliance with the sustainable development goals. In this paper, microbially induced calcite precipitation (MICP) technique was adopted for the bio-cementation of poorly graded standard Ennore sand of India considering relative density of 40%. Adequacy of two urease producing bacteria Sporosarcina pasteurii and Bacillus subtilis has been investigated for the improvement of engineering characteristics of the sand. The duration of treatment is considered to be a 7–14-day period. Monotonic stress–strain behaviour of the MICP-treated sand has been investigated through unconsolidated undrained (UU) triaxial testing. Further, dynamic characteristics (shear modulus and damping ratio) of the treated sand have been evaluated through the use of strain-controlled consolidated undrained (CU) cyclic triaxial tests with loading frequency of 1.0 Hz. The comparative efficacy of both the bacterial treatment in the enhancement of the characteristics of the sand is reported in this study. Moreover, effects of weathering on the characteristics of the bio-cemented samples have been investigated for up to 5 wetting–drying cycles.