This paper introduces a novel thermoacoustic musical instrument, bamboo flute. The system harnesses waste heat to produce sound through thermoacoustic principles, controlling the frequency by opening and closing multiple perforations and achieving the playing effect of a traditional Chinese bamboo flute. Initially, a model of the thermoacoustic flute is developed. Subsequently, an analysis is conducted to assess the impact of the position and number of perforations on thermoacoustic system. The results reveal that the perforation closest to the thermoacoustic core determines the frequency and the distribution of acoustic power. Through manipulation of the perforation boundaries, the system achieves frequency modulation within the range of 277–523 Hz, fulfilling the essential criteria for a G key bamboo flute.