The development of membrane technology sheds light on medical and healthcare industry. At present, medical infusion device mainly relies on glass fiber material to filter bacterial and microparticles from the air entering the liquid medicine. In this Chapter, promising air purifier membranes are exemplified with various material of polyester (PET), polytetrafluoroethylene (PTFE), polypropylene (PP), polyethersulfone (PES) and polyvinylidene fluoride (PVDF) as in medical infusion. Membrane performance including the filtration effectiveness for microparticles removal and the filtration pressure drop for infusion rate is considered respectively. In addition, the protective facial respirators are essential safeguard to prevent microbes transmission. Naturally existing virus range in dimension from 10 to 300 nm. Virus-resistant respirators can be obtained from electrostatic melt-blown filter or modification to the commercial N95 product. Electrospun nanofiber membrane is one of the novel respirator materials and their reusability is tested and analyzed. In addition, the membranes hold an opportunity to advance and implement the ultrafine separation in other medical fields as blood dialysis, artificial heart valves, artificial skin, blood oxygen permeation and so forth.