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In vivo bioimaging involves the non-invasive imaging of internal cells or organs within the human body, offering real-time insights into dynamic biological processes. Its pivotal role in clinical contexts is highlighted by its potential for early disease detection, particularly in critical conditions such as cancer. Despite the existence of established imaging techniques like ultrasound, magnetic resonance imagingMagnetic Resonance Imaging (MRI), and fluorescence imaging, there is an ongoing exploration for innovative imaging probes that can offer enhanced visibility, aiming to deliver high-resolution images. The advent of quantum dots, near-infrared (NIR) fluorescent probes, persistent luminescent nanoprobes, and upconversion luminescent probes for in vivo imaging techniques introduces exciting improvements, ushering in a new era of enhanced bioimaging outcomes. The advent of these nanoprobes offers numerous advantages over traditional fluorescent probes, especially in their capacity to overcome challenges related to light attenuation within biological tissues and autofluorescence. The chapter incorporates sections that elucidate the significance of persistent luminescence and upconversion luminescence specifically in the context of in vivo imaging. Furthermore, the chapter explores the role of various fluorescence imaging techniques in the context of multimodal bioimaging.

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In Vivo Fluorescence Imaging and Its Role in Multimodal Bioimaging

  • 章节

  • 发表日期：

    2025年

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  • Soorya G. Nath,
  • E. I. Anila

摘要

In vivo bioimaging involves the non-invasive imaging of internal cells or organs within the human body, offering real-time insights into dynamic biological processes. Its pivotal role in clinical contexts is highlighted by its potential for early disease detection, particularly in critical conditions such as cancer. Despite the existence of established imaging techniques like ultrasound, magnetic resonance imagingMagnetic Resonance Imaging (MRI), and fluorescence imaging, there is an ongoing exploration for innovative imaging probes that can offer enhanced visibility, aiming to deliver high-resolution images. The advent of quantum dots, near-infrared (NIR) fluorescent probes, persistent luminescent nanoprobes, and upconversion luminescent probes for in vivo imaging techniques introduces exciting improvements, ushering in a new era of enhanced bioimaging outcomes. The advent of these nanoprobes offers numerous advantages over traditional fluorescent probes, especially in their capacity to overcome challenges related to light attenuation within biological tissues and autofluorescence. The chapter incorporates sections that elucidate the significance of persistent luminescence and upconversion luminescence specifically in the context of in vivo imaging. Furthermore, the chapter explores the role of various fluorescence imaging techniques in the context of multimodal bioimaging.