Nanotechnology has significantly advanced, applying the core principles of biological, physical, and chemical sciences at the nanoscale to revolutionize various industries, including medicine. A key focus within this domain is the development and application of nanoscale Metal–Organic Frameworks (MOFs) for medical imagingMetal-Organic Frameworks (MOFs) for medical imaging. MOFs, with their unique structural and functional properties, offer promising enhancements in diagnostic accuracy and treatment outcomes. By manipulating objects at atomic and molecular levels, these nanoparticles, which measure less than 100 nm, exhibit exceptional physiochemical characteristics ideal for bioimaging applications. This chapter delves into the customization of MOFs for medical imaging, examining their potential to provide high-resolution images, improve contrast, and target specific tissues or diseases with precision. Through this exploration, we aim to underscore the transformative impact of MOFs in medical imaging, highlighting their role in advancing diagnostic techniques, enhancing therapeutic interventions, and contributing to economic growth and social welfare by improving patient care and medical research.