This study was geared to find out areas of knowledge possessed by staffs that carry out reference and information (RIS) in Federal University libraries in the lower Southern region of Nigeria, comprising six states. The basis for the existence of university libraries was discussed and its driving force being information and communication technologies. The paper also noted that Information technology has become the mainstream in library services generally. Two structured questionnaires were deployed, one of which was to gather data from 193 staff who offer core reference and information services alongside allied reader services in the selected university libraries. The secondquestionnaire was for sectional/ unit heads of reference services.The first questionnaire for RIS staff (including those in Reader services), was to determine the extent of their knowledge of technological applications for performingthe RIS. The overall mean rating in all the responses indicated their knowledge in this respect was to a ‘great extent.’ The following rated highest: knowledge to send and receive e-mails with overall meanof 3.13, knowledge of search engines’ with overall mean rating of 3.11 followed by knowledge to provide assistance in the use of E-resources - 3.09, knowledge of the use of E-catalogues with 3.06 overall mean, knowledge of tools for E-reference service by 3.02 andknowledge of various non-print resources by 3.02 overall mean. The second questionnaire with findings from heads of sections also indicated electronic reference and information services (ERS) carried out in the selected university libraries, was to a great extent. In view of this outcome, it was recommended among others, that social media profiles for libraries should stay active at corporate level. Also systems maintenance and other technical operations should not be relinquished to engineers only, but to librarians.