This chapter discusses in detail the defense training form of cybersecurity training. An overview of the defense training methodology is provided first, focusing on the interdependency with respect to the other forms of cybersecurity training, namely attack and forensics training. The two main defense training types, fundamental defense training and defense methodology training, are presented next, examining in detail the various methods used to teach or learn basic defense skills, as well as the overall defense methodology. A set of additional resources is also provided, such as knowledge bases (D3FEND, Engage, CAR, etc.) and defense methodology guidelines, as well as defense training tools (iptables, OpenVAS, Snort) and platforms. Finally, the main advantages of defense training, as well as potential issues that may arise, are discussed, both from the trainee and organizer perspectives.