MXene is a novel material composed of early transition metals and relatively light elements like carbon and nitrogen with layered structure similar to graphene and extensively studied in many fields, such as catalysis, sensors, energy, and electronics. MXene has many characteristic features such as high dispersibility in polar solvents, optical properties, and high conductivity. Because of the high conductivity, MXene is expected to improve the functions of electrode materials. This chapter focuses on the electronic properties of MXene and the applications to electrode materials of fuel cells, especially, biofuel cells. At first, as a background, researches related to fuel cells and their electrode materials are introduced. In the next section, preparation and the physical properties of MXene, such as morphology, particle size, thermal stability, and electronic properties, are covered. As the following topics, this chapter deals with the electronic properties of MXene as electrode materials, along with practical examples and comparisons with other electrode materials such as carbon nanotubes. This section also deals with the applications of MXene as anode and cathode materials for biofuel cells. In the summary section, an overview of applications for electrodes and advantages as electrode material of MXene in biofuel cells and the expected future trends are mentioned.