The present chapter aims to give a multifaceted view of the biobased barrier materials suitable for active food packaging. To begin with, barrier functions for mass migration of moisture, gas, odor, and microbes and controlled release of active constituents from package to food are addressed along with energy barrier features like light/irradiation trap and heat insulation. It is followed by exploring various biomaterials used for delivering barrier functions. This context explores polysaccharides, proteins, lipid coatings, fermentation-derived polylactic acid and polyhydroxyalkanoates, food wastes, their derivatives, and green nanomaterials. The factors affecting the features of barrier materials and the latter’s suitability for active packaging are critically evaluated from consumer acceptability, health and safety, food properties, and active material compatibility perspectives. Moreover, multilayering techniques to overcome poor barrier properties of biopolymers are elaborated. With this, this chapter provides a solid understanding of the challenges and potential of biobased barrier materials for active packaging.