Application of Biotechnological Tools in the Abies Genus: An Overview about the Application of Somatic Embryogenesis and Other Cloning Techniques
摘要
In the last decades, plant cloning took a prominent place in the propagation of many tree species and proved its importance to achieve large-scale purposes. Somatic embryogenesis (SE) is a biotechnological tool, which has been applied to propagate many conifer species with many advantages. This technique is used in the intensive propagation of many conifer species and, especially, of Christmas trees because of its high demand in the market. For the genus Abies, this technique was initiated decades ago and still it has many challenges to improve its application to attain the commercial level. As many other conifers, diverse Abies species are threatened by climate change, deforestation, wildfires, livestock grazing, and genetic drift, making the improvement of its preservation and multiplication of utmost relevance. Therefore, the optimization of SE protocols could be a crucial tool to its propagation. In this review, the achievements of SE in the Abies genus to date are reported, with a focus on the use of the embryogenic tissue (ET) for cryopreservation, genetic advances, and other research studies.