Terahertz (THz) waves exhibit important biological effects due to their unique interactions with biological macromolecules. THz wave can be directly coupled with biomolecules to produce non-thermal effects and excite the nonlinear resonance of biomolecules, which can lead to structural or conformational changes of biomolecules, resulting in a series of macroscopic biological effects. Here, we find that terahertz waves of specific frequencies can alter energy metabolism in T cells. Terahertz wave can promote the further utilization of glucose by T cells. Without affecting the rate of glycolysis, more glycolytic products enter the tricarboxylic acid cycle to improve the utilization of glucose by T cells. In addition, we also found that after terahertz stimulation, the glucose uptake of T cells decreased, and the content of key intermediate products in the tricarboxylic acid cycle increased, these results indicate that T cells can use less glucose to meet their normal energy metabolic activities and improve the energy utilization of T cells.