Industrial agriculture is trying to fulfill the growing food demand of the fast-increasing population. In pursuit of this, synthetic chemicals are extensively used in terms of pesticides, insecticides, fertilizers, hydrocarbons which employ harsh synthetic surfactants having harmful effects on the microenvironment of soil and ecosystem due issues of non-biodegradability and toxicity associated with them. Biosurfactants of microbial, animal or plant origin are eco-friendly and may replace synthetic and chemical surfactants. Biosurfactants are significant in formation of biofilm, dealing with the plant pathogen, bioremediation, biodegradation, and enhancing the bioavailability of nutrients in the soil for the plants. At present, potential biosurfactants are basically surfactin, rhamnolipid and sophorolipids derived from B. subtilis, P. aeruginosa, T. bombicola, respectively. Biosurfactants are extremely helpful with bioremediation of heavy metals by enhancing their mobility and facilitating complex formation through emulsification and adsorption. Biosurfactants enhance the bioavailability of various elements and micronutrients to the soil due to formation of cation–anion complexes and solubilization facilitating their absorption by roots of the plants. Biosurfactants cause wetting and thus find promising applications as emulsifiers which are extremely useful in the pesticide industry. They also antagonize plant pathogens naturally and thus can be developed as biopesticides to promote pest management in a sustainable way. Biosurfactants which are in their infancy need to be carried to the next level in terms of their production, extraction in an economically viable way for the maximum benefit in the era of industrial agriculture. Genetic engineering may help in modifying biosurfactants extraction befitting the specific requirements and exploring new horizons. There is also a need for evaluating the repercussions of biosurfactants on soil and crops in long run, in terms of microbiome, their capacity for nutrient absorption, utilization for agricultural sustainability. Biosurfactants are associated with the bioremediation of the heavy metals and hydrocarbon pollutants in the soil or the oil spill. The biosurfactants are less toxic, biodegradable, highly active and stable in the temperature and varying environmental conditions. These biosurfactants are a promising help in promoting green technology in the era of industrial agricultural. Soil quality is improved by flushing, mobilization, and solubilization of nutrients. Biosurfactant may play a key role in sustainable development in next generation of agriculture which may be achieved by extensive commercialization, uses, enhancing their affordability.