The global infrastructure sector needs sustainable alternatives to conventional materials, particularly bitumen derived from crude oil refining, which has long been integral to road construction. However, its use contributes to environmental degradation, finite resource depletion, and economic volatility. Concurrently, reducing global carbon emissions has become imperative, compounded by projections of a growing human population and increased infrastructure demand. Rising crude oil prices have further underscored the necessity for renewable construction alternatives, prompting exploration into bio-based options sourced from industrial bio-waste. This study uses material characterization and laboratory testing to explore the practicality and effectiveness of bio-binders obtained from ethanol lignin and bio-residue, employing either chemical or bio-based additives. The research also underscores the potential of bio-binder to contribute to the sustainability of bituminous pavements by replacing a portion of the binder.