Patients with malignant bone tumors require surgical resection, chemotherapy, and radiation therapy. To cure the disease, surgical tumor resection with a sufficient surgical margin is necessary. After the tumor resection, the bone defects require reconstruction using tumor prosthesis or bone graft, including iliac or fibular bone graft, allograft, or devitalized tumor bone graft treated with irradiation, liquid nitrogen, or pasteurization. Although tumor prosthesis is widely used due to the simple procedure and early rehabilitation, the long-term outcomes and functional outcomes are unsatisfactory due to difficulty in reattachment of tendons and muscles to the tumor prosthesis. On the other hand, biological reconstruction can be expected to have long-term durability due to revitalization. Furthermore, good limb function can be achieved through adhesion of muscles and tendons. Among biological reconstructions, tumor-bearing autografts treated with liquid nitrogen have several advantages, including good osteoconduction, osteoinduction, simple procedure, perfect fit, low cost, and preservation of bone morphogenic proteins. In this section, several procedures of tumor-bearing autografts treated with liquid nitrogen are described.