Recent notable advances in three-dimensional (3D) printing have significantly transformed the design and fabrication of microneedle (MN) devices, offering unprecedented control over geometry, material selection, and functional integration. This chapter presents an overview of the general concepts underlying 3D printing in microneedle technologies, with a focus on state-of-the-art 3D printing techniques. Key printing methods, including stereolithography, digital light processing, and two-photon polymerization, are discussed in terms of their working principles, resolution capabilities, and suitability for MN fabrication. The chapter further explores emerging biomaterials such as smart polymers, bioinks, and composite materials that enhance mechanical performance, biocompatibility, and drug delivery functionality. Advances in computer-aided design tools, including AI-assisted design, topology optimization, and simulation software, are highlighted as critical enablers of precision and customization in MN development. Lastly, limitations and challenges related to scalability, material constraints, and regulatory considerations are discussed, providing insights into future directions for 3D-printed microneedle systems.