The concept of surface roughness is intuitive: it can be associated with a sensory perception describing the adhesion and sliding characteristics of a surface. Roughness is an essential element to understand the physical characteristics of various surfaces and their evolution. Nowadays, in many application fields, we use a variety of textile surfaces. These are distinguished by their geometric and structural parameters at three levels: the microscopic level (fiber morphology, fibrous level), the mesoscopic level (yarn level), and the macroscopic level (knit level). The evaluation of surface roughness is crucial in many areas: surface finish, knitwear quality, and perception of garment comfort. The objective of this article is to investigate the factors affecting the surface of knitwear during storage in order to better understand their impact on subsequent production phases. To achieve this objective, we carried out the evaluation of the surface properties of knitwear by the Kawabata warp surface module “KES- FB4”, focusing on the effect of relative humidity, storage modes, as well as the type of knit structure (jersey, rib and fleece knits) and its composition (cotton, viscose and polyester) on this phenomenon. Cellulose-based knitwear stored in a high humidity environment becomes rougher (less smooth), due to the increase in the linear thickness deviation (SMD). It was found that the textile composition, environmental conditions and storage conditions and configurations have an impact on the surface properties of the knitwear studied. This study could be of great use in predicting the behavior of knitwear in the different phases of production, marketing and use; thus, allowing to predict and define the optimal conditions to be given to the knitwear in these phases to ensure: dimensional stability, comfort and durability.