Twin-related domains are formed through multiple twinning operations that originate from a single nucleus. Statistical analysis reveals that these domains exhibit several dominant orientations, with a small number of these orientations comprising the majority of the area and grains within a domain. Most misorientations between these dominant orientations demonstrate low-order ∑3n-type (n = 1, 2), and the four sub-dominant orientations typically represent twinning variants of the first-dominant orientation. These statistical characteristics demonstrate two general behaviors of multiple twinning: back-and-forth patterns and preferential orientations. Twinning operations generate higher (forward) and lower (backward) generation orientations, with the probability of backward twinning exceeding that of forward twinning. Multiple twinning tends to result in certain preferential orientations, leading to dominant orientations within the formed twin-related domain. A novel metric called the through-view random boundary path (TRBP) was introduced to evaluate random boundary connectivity. The TRBP represents a chain of end-to-end connected crack-susceptible boundaries that traverse the entire mapped microstructure. Following twin-related domain growth mechanisms, connected non-twin boundary networks persisted in the grain boundary engineering (GBE) sample regardless of the twin boundary fraction. An increasing in the twin boundary fraction corresponded with a decrease in TRBP numbers and a monotonically increasing normalized minimum TRBP length, suggesting improved resistance to intergranular degradation.