It was known which of the cis, medial, and trans cisternae in the Golgi apparatus corresponded to the localization of glycosyltransferase, but it was not well understood where it was localized within each cistern. The main reasons for this were the lack of appropriate antibodies to indicate the localization of glycosyltransferase and the lack of spatial resolution of the optical microscope used to observe the Golgi apparatus. Therefore, we introduced tags such as c-myc at the C-terminus of the glycosyltransferase genes by knock-in using the CRISPR/Cas9 method, making it possible to visualize the localization of these glycosyltransferases. Furthermore, using super-resolution microscopy, which has been developed in recent years, we clarified the detailed localization of these enzymes [1] (Fig. 29.1). As a result, we found that the Golgi apparatus is composed of basic structures called Golgi units, which are 1–3 microns in diameter, and that Golgi units undergo dynamic processes such as division and fusion. Glycosyltransferases were found to be concentrated as clusters within the Golgi units which we named this the glycosyltransferase zone. The zone was localized inside the Golgi unit, especially at its periphery. Furthermore, the localization of the N-type and O-type glycosyltransferase zones was almost the same, but the proteoglycan synthesis enzyme zone was smaller and located differently. In addition, it was found that these zones move very quickly around the edge of the Golgi unit.