This volume classifies the current challenges in glycoscience into six core parts. Within each part, specific themes were assigned to experts with specialized knowledge, who provided their insights through a structured questionnaire. Each theme was evaluated from six key perspectives: current status, impact, basic research, applicability, future prospects, and challenges. This comprehensive overview, summary and future perspectives in glycoscience, critically examines the findings from each part and provides a comprehensive analysis of the current landscape and future directions in glycoscience. By integrating expert knowledge across diverse fields, it serves as both a status report and a strategic guide for the continued advancement of glycoscience research and innovation.