Many pharmaceuticals have a chiral structure, and because the pharmacological activity and side effects differ between enantiomers, there is a strong demand for the use of more useful enantiomers. For this, efficient methods of obtaining both enantiomers and trace analysis methods are needed [1, 2]. The separation of enantiomers by high-performance liquid chromatography (HPLC) or supercritical fluid chromatography (SFC) is attracting attention as a method that can be adapted to both the enantioseparation and analysis of enantiomers (Fig. 151.1). The key to these methods is a chiral stationary phase with high chiral recognition ability, and various chiral stationary phases have been reported so far, with more than 200 types already on the market. Among them, the most commonly used today are phenylcarbamate and benzoate derivatives of cellulose and amylose [3–5]. Many low-molecular-weight compounds and polymers with chiral recognition ability are used as chiral stationary phases, but none have been found to match the abovementioned polysaccharide derivatives.