In most higher eukaryotes, the coding regions (exons) of multi-exon genes are separated by noncoding regions (introns). RNA polymerase II cannot distinguish between coding and noncoding regions during transcription. Instead, it transcribes the entire sequence of the target gene into a precursor messenger RNA (pre-mRNA), which lacks translational functionality. The cell must remove the noncoding introns through RNA splicing and connect the coding exons to produce a mature mRNA molecule capable of directing protein synthesis. During RNA splicing, the pre-mRNA transcribed from the same gene can undergo different splicing mechanisms and exon combinations, resulting in mRNA isoforms that encode proteins with distinct functions. These isoforms are referred to as alternative splicing variants (ASVs) or splice isoforms, and the process is known as alternative splicing (AS) [1, 2].