RNA interference (RNAi) and double-stranded RNA (dsRNA)-mediated gene silencing have evolvedinto novel, eco-friendly approaches for combating the viral diseases of fruit crops. Plant viruses are responsible for a great reduction in the yield andquality of fruits. Conventional methods of control, suchas insecticidal treatment and vector control, are frequently inadequate or lacking in specificity. RNAi takes advantage of the intrinsicdefense systems of the plant, in which viral RNA is targeted for destruction through sequence-specific gene silencing. Such regulation may be provided by transgenic expression ofvirus-derived dsRNA in the plant or non-transgenic applications outside the plant of synthetic or in vitro produced dsRNAs. The development of advanced dsRNA application techniques, including foliar sprays, trunk injections, as well as nanoparticle carriers, has now made the use of RNAi possible in fruit crops, which are commonly perennial having limited genetic transformation capabilities. These strategies can successfully stifle viral genes, lower the frequency of virus particles, and provide the plantwith resistance without affecting the genome. Indeed, a few reports have shown RNAi-driven immunity against fruit-infecting viruses, such as papaya ring spot virus (PRSV). The evolution of bioinformatics also contributed to the development of RNAi products, wherehighly specific dsRNAs can be designed with minimal off-target effects. RNAi and dsRNA-based gene silencing techniques provide a sustainable, targeted, and safe approachfor virus management in fruit crops that could potentially transform plant protection and strengthen worldwide fruit production systems, as discussed in this book chapter.