Amorphous alloy 3D wound core transformer has significant energy-saving advantages, but its hysteresis effect far exceeds that of silicon steel transformers. To analyze the influence of insulating oil temperature on transformer vibration, the vibration characteristics of tank under different insulating oil temperatures is studied. Firstly, an experimental platform for the magnetic properties of amorphous alloy 3D wound core is established to obtain basic magnetization data. Secondly, based on the theory of elastic mechanics, a magneto-mechanical vibration coupling mathematical model is constructed. Finite element simulation calculations are carried on the 3D model of a 50 kVA transformer to verify the accuracy of the finite element method, and obtaining the vibration distribution of the tank under room temperature conditions. Finally, the experiment is carried out on the vibration characteristics of amorphous alloy 3D wound core transformer under different insulation oil temperatures. The vibration trend of the tank under varying insulation oil temperatures is obtained, revealing the influence of insulating oil temperature on the vibration characteristics of the transformer and providing theoretical support for further reducing the vibration and noise of amorphous alloy 3D wound core transformer.