Considering the electrical anisotropy of the cell membrane, analyze the factors affecting cell electrical rotation. A single shell cell physical model with anisotropic membrane was selected as the research object. The Clausius Mosotti factor was theoretically analyzed, and the influence of cell geometric and electrical parameters on its electrical rotation was numerically analyzed using MATLAB software. Cells undergo electrical rotation under the action of a time-varying electric field. The threshold frequency of the first and second co rotation is positively correlated with cell membrane thickness and radial membrane conductivity, and negatively correlated with extracellular radius. The first co rotating angular velocity is negatively correlated with cell membrane thickness and radial membrane conductivity, and positively correlated with outer radius, The second co rotating angular velocity is positively correlated with cell membrane thickness and negatively correlated with extracellular radius and radial membrane conductivity, The reverse rotation threshold frequency is negatively correlated with the relative dielectric constant of intracellular fluid and positively correlated with the relative dielectric constant of extracellular fluid, The tangential membrane conductivity does not affect the rotation of cells in an electric field. By analyzing the factors that affect the electrical rotation of anisotropic cells, we can gain a deeper understanding of the physiological state of cells and provide some theoretical basis for biophysics and cell biology.