Transcranial focused magnetoacoustic electrical stimulation (TFMAES), as a novel non-invasive brain neuromodulation technique, demonstrates significant potential for application. To investigate the interventional effects and potential amelioration of Alzheimer’s disease (AD) symptoms by this technique, we adopted a research strategy integrating theoretical simulation with animal experiments. Employing the finite element method, we simulated the spatial distribution characteristics of both the static magnetic field used for excitation and the acoustic field within the target brain region during stimulation. Subsequently, we derived the spatial distribution of the induced current. In vivo experimental validation was performed using transgenic AD model rats. Through a series of behavioral tests, the behavioral performance changes of rats in cognitive function and learning memory ability before and after TFMAES intervention were quantitatively evaluated. Collectively, the experimental results demonstrated that TFMAES significantly improved cognitive impairments and deficits in learning and memory in AD rats, thereby providing an important experimental basis for its use as a new strategy for AD treatment.