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The book chapter demonstrates how CRISPR/Cas12a genome editing technology enables agricultural breakthroughs that enhance food security. The worldwide food supply depends heavily on grains, but these crops encounter two major threats from climate change and pathogenic attacks. CRISPR-/Cas12a-based biosensors provide an efficient, portable, and affordable solution to fight these threats through their operation beyond conventional methods. The CRISPR/Cas12a system performs trans-cleavage through its guide RNA (crRNA), which guides the system to recognize particular DNA sequences. The system generates fluorescent or colored signals through probe cleavage, which occurs when the activity is activated. The system functions as a biosensor to detect nucleic acid contaminants and non-nucleic acid substances, including mycotoxins, pesticides, heavy metals, and bacteria. The detection capabilities of CRISPR/Cas12a biosensors surpass those of conventional methods because they provide better specificity and sensitivity. The technology will merge with the Internet of Things (IoT) and artificial intelligence systems to create better crops that fight diseases and stresses while ensuring safer food distribution throughout the supply chain. The combined systems enable real-time monitoring and transparency to stop food fraud and defend public health through integrated capabilities.

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CRISPR/Cas12a-Based Biosensors and Applications in Cereal Safety

  • 章节

  • 发表日期：

    2026年

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  • Fatma Nur Basturk,
  • Seran Kırkıncı,
  • Nazlıcan Yıldırım,
  • Yiğit Sabri Unlu,
  • Hande Morgil,
  • Emirhan Çağla,
  • Berke Çevik,
  • Kadir Boztas,
  • Gül Cevahir Öz,
  • Yusuf Can Gerçek

摘要

The book chapter demonstrates how CRISPR/Cas12a genome editing technology enables agricultural breakthroughs that enhance food security. The worldwide food supply depends heavily on grains, but these crops encounter two major threats from climate change and pathogenic attacks. CRISPR-/Cas12a-based biosensors provide an efficient, portable, and affordable solution to fight these threats through their operation beyond conventional methods. The CRISPR/Cas12a system performs trans-cleavage through its guide RNA (crRNA), which guides the system to recognize particular DNA sequences. The system generates fluorescent or colored signals through probe cleavage, which occurs when the activity is activated. The system functions as a biosensor to detect nucleic acid contaminants and non-nucleic acid substances, including mycotoxins, pesticides, heavy metals, and bacteria. The detection capabilities of CRISPR/Cas12a biosensors surpass those of conventional methods because they provide better specificity and sensitivity. The technology will merge with the Internet of Things (IoT) and artificial intelligence systems to create better crops that fight diseases and stresses while ensuring safer food distribution throughout the supply chain. The combined systems enable real-time monitoring and transparency to stop food fraud and defend public health through integrated capabilities.