Plant diseases play a crucial role in agriculture since many of them affect a variety of food crops, including vegetables, fruits, and basic grains, lowering their quality and productivity. With enhanced yield, quality, and abiotic/biotic stress tolerance, the CRISPR/Cas systems hold promise as a valuable genome-editing technique for disease detection and the development of new cultivars of crops resistant to pathogens. This method can assist in the early diagnosis of various viral, fungal, and bacterial infections, enabling the prompt application of plant medicines to stop the spread of those diseases more effectively. A promising strategy for the quick and precise identification of pathogens is the creation of a diagnostic tool for plant diseases based on CRISPR-Cas systems. By enabling prompt interventions and lowering crop losses, this instrument has the potential to greatly enhance plant disease management. For this technology to be validated and improved for practical application in agricultural contexts, additional study and improvement are still needed. Together with CRISPR/Cas technology, next-generation colorimetric sensors can overcome these obstacles and develop into highly effective and user-friendly diagnostic instruments. This review will provide insights into a variety of CRISPR-based diagnostic tools, their applications in plant disease, challenges, and the future promise of the CRISPR-Cas system.