This study focuses on parametric variation of producing biodiesel from unrefined and refined palm oil using transesterification to achieve high yields. Palm oil is first converted to fatty acid methyl esters (FAME) through transesterification, with production varying based on temperature, alcohol ratio, and stirring time. FAME is then blended with diesel to create biodiesel blends (B5 to B50). The properties of the biodiesel blends are analyzed with respect to fuel composition and energy content. With optimal parameters of 60 min stirring time, 60 °C temperature and alcohol-to-oil ratio of 6:7, we a conversion of 99.5% refined palm oil to biodiesel was achieved. This sample not only results in a high conversion rate of 99.5% but also boasts a calorific value of 40,051 kJ/kg. From the elemental analysis, the POBDs are mainly built up by stearic and palmitic acid, which accounts for over 96% of its content. With the optimal result, the POBD was blended with petroleum diesel to form B0 to B50 with 5% incremental. The resulting calorific values were 46,317 and 42,055 kJ/kg respectively. Adding 5% volume of POBD with petroleum diesel decreases 1% of the blend’s calorific value.