The predictors of maternal gatekeeping—the mother's behaviors that regulate the father's involvement in childcare—are still not well established. This study aimed to explore relationships between maternal gatekeeping – both gateclosing and gateopening behaviors, and mothers’ perception of father competence in childcare and mothers’ negative emotionality traits. A sample of 100 Thai mothers from dual-income different gender relationships reported their gatekeeping behaviors, perception of father competence, and negative emotionality traits. A standard multiple regression revealed that mothers were more likely to engage in maternal gateclosing when they perceived lower father competence and possessed a higher negative emotionality trait. They were more likely to engage in maternal gateopening only when fathers were perceived as more competent. No significant relationship was found between maternal gateopening and mothers’ negative emotionality trait. The findings contributed to the limited literature on maternal gatekeeping, pioneered the research in Thailand, and informed intervention strategies.