The ability of entrepreneurial start-ups to foster a sustainable entrepreneurship orientation (SEO) into their business practices and culture is yet to be fully explored in emerging countries. This chapter examines the influence of critical drivers of SEO in South African start-ups. Using empirical data from 220 manufacturing start-ups, structural equation modeling was employed to test the influence of three driving factors, namely individual factors, organisational factors, and contextual factors, on SEO. Results show that all three driving factors influence SEO. However, findings illustrate that contextual factors are the most influential drivers in influencing start-ups to engage in SEO. The chapter concludes that cultivating SEO is not solely a function of personal aspiration or corporate strategy; it necessitates a comprehensive approach that incorporates multiple elements affecting entrepreneurial conduct. This chapter enriches the literature on SEO in emerging economies. The chapter has significant implications for policy makers on the establishment of supportive regulatory frameworks, and incentives that encourage start-ups to adopt sustainable practices. The chapter illustrates how entrepreneurial orientation and sustainability can coexist, validating that start-ups can incorporate environmentally friendly practices into their business models. This contributes to the goal of promoting resource efficiency. This aligns with SDG-13.