Entomopathogenic fungi (EPF) are crucial regulators in ecosystems, influencing host populations and biodiversity. Despite their ecological significance and potential as biocontrol agents, our understanding of EPF remains limited due to gaps in knowledge related to taxonomic clarity, host specificity, and geographical distribution. However, advancements in molecular techniques, including DNA barcoding, multilocus sequencing, and whole-genome sequencing (WGS), are transforming EPF classification. These methods are overcoming the limitations of traditional morphology, revealing previously hidden diversity and intricate phylogenetic relationships across major fungal phyla. Hypocreales (Ascomycota) is the most prominent among EPF, comprising key genera such as Metarhizium, Beauveria, and Ophiocordyceps. Comparative studies indicate that ecological factors, rather than host specificity alone, often drive speciation. Therefore, an integrative taxonomic approach, combining morphological, ecological, and genomic data, is critical for accurate species identification, understanding host-pathogen dynamics, predicting species invasions or extinctions, and optimizing EPF for sustainable pest management and ecological conservation.