Oxidative stress, resulted from the excessive generation of reactive oxygen species (ROS), contributes in skin aging by damaging cellular macromolecules. Oral supplementation with antioxidant-rich formulations has emerged as a promising approach to counteract skin aging by reducing oxidative stress. TriGlow+ is a health beverage composed of collagen tripeptide, multiple fruit extracts (lemon, apple, carrot, grape seed, green tea, aloe vera, Garcinia atroviridis), and glutathione, which collectively possess antioxidant and anti-aging potential. This study aimed to evaluate the anti-aging and antioxidant activity of TriGlow+. Antioxidant capacity was assessed by DPPH and FRAP assays, while anti-aging activity was measured through elastase and collagenase inhibition assay. DPPH assay revealed dose-dependent free radical scavenging activity with an IC₅₀ of 3014.68 ± 146.82 µg/mL. In contrast, FRAP results demonstrated strong ferric ion reducing power, with values elevated at 50–100 µg/mL (257.55 ± 2.85 and 282.58 ± 2.44 µM Fe (II)/µg). TriGlow+ also inhibited key aging-related enzymes in a concentration-dependent manner. Elastase inhibition IC₅₀ is 46.19 ± 0.31 µg/mL, indicating strong inhibitory potential. Collagenase inhibition exhibited IC₅₀ value of 182.10 ± 1.96 µg/mL, showing moderate efficacy. In conclusion, TriGlow+ exhibits notable antioxidant and anti-aging activities, particularly through ferric reducing power and inhibition of elastase and collagenase. Overall, the study provides foundational evidence supporting the functional benefits of TriGlow+. These findings highlight TriGlow+ as a promising oral nutraceutical for promoting skin health and combating oxidative stress, warranting further investigation into its active components and clinical efficacy.