This study was designed to compare the effect of Eccentric and Concentric training along with ultrasound on hamstring tightness using a Digital Goniometer in amateur football players. A total of 50 subjects were selected and divided into two groups that included Eccentric training group (n = 25) and Concentric training group (n = 25). The Digital goniometer and Sit and Reach test were used to evaluate the functional activity and range of motion. Both interventions were given for six weeks, five sessions per week for 40 min. The paired and unpaired tests were used to analyze the values. The pretest and post-test values were analyzed by using Digital goniometer application and Sit and Reach test in both groups. The mean value of the Eccentric training group was found to be more significant than the Concentric training group with a p value of <0.0001. The study revealed that Eccentric training was found to be more successful in functional activity and range of motion than Concentric training for hamstring tightness.