Aiming at the technical difficulties of efficient filling and emptying system, anti-cavitation design for filling/emptying valves, and adequate energy dissipation in the lock chamber posed by the construction of high head and large-scale locks in the lower reaches of the Jinsha River, a 60 m working head single-lift lock filling and emptying system was proposed, the arrangement scheme of inertia-equilibrium filling and emptying system with two-section four-manifold and three-dimensional layered energy dissipation. The cross-sectional dimensions of the the filling and emptying system culverts under this scheme were calculated, detailed configurations of energy dissipators and the upstream and downstream inlet types were comprehensively analyzed. By establishing a mathematical simulation model of the lock filling and emptying process, hydraulic parameters such as inertial length and resistance coefficient were estimated. The hydraulic characteristics of the filling and emptying process were analyzed, and the valve opening strategy was recommended. The calculation results show that under the recommended opening time of the filling and emptying valves, the filling and emptying time of the lock can meet the design requirements, and other hydraulic indicators of the filling and emptying system meet the specifications. The calculation results verify the feasibility of the layout of the 60 m level single-lift lock filling and emptying system, providing theoretical support and scientific basis for the planning of navigation facilities in the lower reaches of the Jinsha River.