Radish (Raphanus sativus L.) is an annual or biennial root vegetable with short cultivation cycle belonging to the Brassicaceae (mustard) family. Apart from its culinary uses, radish holds medicinal value, attributed to its bioactive compounds like glucosinolates, flavonoids, and anthocyanins. Radish faces various biotic stresses, including fungal, bacterial, and viral pathogens, insect-pests, and nematodes significantly affecting the yield potential. These biotic stresses cause physiological disruptions, yield losses, and reduction in quality. Host overcomes the attack of pathogens and insects by deploying resistance mechanisms including constitutive defenses, viz., physical barriers and antimicrobial compounds, and inducible defenses like systemic acquired resistance (SAR) and induced systemic resistance (ISR) pathways. Successful breeding for resistance against these biotic stress factors involving conventional breeding methods augmented by marker-assisted selection (MAS) has been shown to be effective in developing resistant varieties. However, the advent of biotechnological tools like transgenics, RNA interference, and CRISPR/Cas9 genome editing offers futuristic solutions that provide broad-spectrum and durable resistance. Integrated pest and disease management (IPDM) strategies encompassing cultural, biological, and chemical measures combined with host resistance emphasize sustainable biotic stress management in commercial radish cultivation. Future research leveraging genomics and bioinformatics holds promise for enhancing radish resistance against biotic stresses and securing its agricultural viability.