Nanotechnology is rapidly emerging as a transformative force in agricultural practices, particularly in the field of genetic engineeringGenetic engineering, offering solutions for improving crop resilience, yield, and sustainability. By integrating nanomaterialsNanomaterials with cutting-edge geneGene-editing toolsEditing tools, such as CRISPR/Cas9CRISPR/Cas and Prime Editing, plant-based nanotechnology is providing precise and efficient ways to address global challenges, including food security, climate changeClimate change, and environmental stress. Silver nanoparticlesSilver nanoparticles (AgNPs), zinc nanoparticles (ZnNPs), carbon nanotubesCarbon nanotubes (CNTs), gold nanoparticlesGold nanoparticles (AuNPs), and lipid nanoparticlesLipid nanoparticles (LNPs) are key players in this technological revolution, enhancing the delivery of genetic material into plant cells, improving transformation efficiency, and increasing plant stress toleranceTolerance. These nanomaterialsNanomaterials also contribute to enhanced pathogenPathogens resistance and overall plant health, making them invaluable in the development of crops that can thrive in challenging environmental conditions. The use of next-generation sequencing (NGS) technologiesTechnology further amplifies the potential of these tools by providing high-throughput genomicGenomics analysis that enables the precise monitoring of genetic modifications. As these technologiesTechnology evolve, plant-based nanotechnology holds the promise of sustainable agriculture by minimizing chemical inputs, improving resource efficiency, and enabling the development of crops better suited to environmental stresses. This review explores the latest advancements in plant-based nanotechnology, focusing on its applications in plant genomicsGenomics and genetic engineeringGenetic engineering, and discusses its potential to revolutionize the future of agriculture.