Plants are constantly exposed to a wide array of environmental stressors-ranging from abiotic factors like drought, salinity, extreme temperatures, and heavy metalsHeavy metals to biotic threats such as pathogensPathogens and herbivores. These stress conditions disrupt normal metabolic processes and significantly impact plant growthPlant growth and productivity. MetabolomicsMetabolomics, the comprehensive study of small-molecule metabolites, provides a direct snapshot of a plant’s biochemical state during stress. However, traditional metabolomicMetabolomics tools often fall short in sensitivity and real-time analysis, especially when tracking low-abundance or rapidly changing metabolites. The integration of nanotechnology into metabolomicsMetabolomics-referred to as nano-metabolomicsNano-metabolomics-has emerged as a transformative approach to overcoming these limitations. Engineered nanomaterialsNanomaterials such as gold nanoparticlesGold nanoparticles, carbon nanotubesCarbon nanotubes, graphene, and quantum dotsQuantum dots exhibit unique properties that dramatically enhance metabolite detection, sampling efficiency, and in vivo monitoring. With the aid of nano biosensors, microfluidic devices, and surface-enhanced techniques, researchers can now detect stress biomarkers like proline, abscisic acid, and volatile compounds with remarkable precision-even in trace amounts. This chapter highlights the powerful synergy between nanotechnology and plant metabolomicsPlant metabolomics, showcasing how nano-enabled platforms unravel the metabolic reprogramming associated with various stress conditions. From monitoring osmolytes and antioxidants under drought or salinity to profiling defense compounds during pathogenPathogens attacks, nano-metabolomicsNano-metabolomics opens new frontiers in plant stress biology. Case studies further illustrate real-world applications, including wearable nanosensors for drought monitoring and nanoparticle treatments that boost plant resilience. Emerging nano-tools-coupled with artificial intelligence and digital farming technologiesTechnology are redefining the future of crop monitoring and stress diagnostics. While challenges like nanotoxicity, environmental safety, and standardization persist, the potential of nano-metabolomicsNano-metabolomics to revolutionize sustainable agriculture and precision crop management is immense.