Carbon dots (CDs) are carbon-based nanoparticles that have emerged as nanomaterials, possessing physicochemical, optical, and biocompatible properties. Carbon dots are a family of fluorescent nanoscale materials that behave like quantum dots, usually between 1 and 10 nm in diameter. Due to their fluorescence, lower toxicity, water-solubility, tunable emission across a broad range of wavelengths, and ease of modification properties, they hold great potential for applications in bioimaging, biosensing, medicine, drug delivery, electrocatalysis, and photocatalysis. Recently, green synthesis procedures have arisen as sustainable and environmentally acceptable alternatives to traditional chemical methods, utilizing natural precursors such as herbal extracts, biomass, agro-waste, and organic debris. These approaches have provided benefits like cost-efficiency and affordability, low toxicity, and a lesser impact on the environment. This chapter highlights the synthesis of green carbon dots, their synthesis methods, and beneficial characteristics resulting from animal-, plant-, and agro-waste-based resources and possible applications in biomedicine.