Fungal infections in animals pose a significant challenge to veterinary medicine, with species such as Aspergillus, Candida, and Cryptococcus causing severe opportunistic and systemic infections. Conventional antifungal treatments, including polyenes, azoles, allylamines, and echinocandins, present several limitations, such as narrow–spectrum activity, drug resistance, toxicity, and poor pharmacokinetics. These challenges necessitate the exploration of novel therapeutic strategies, including the use of nanoparticles. Nanomaterials offer unique physiochemical properties, such as enhanced bioavailability, targeted drug delivery, and antifungal mechanisms that overcome conventional drug resistance. This chapter provides an overview of fungal infections affecting animals, the drawbacks of existing antifungal therapies, and the emergence of nanotechnology as a promising alternative. Various classes of nanoparticles, including metal and metal oxide nanoparticles, polymeric nanoparticles, lipid–based nanoparticles, and carbon–based nanoparticles, have been investigated in the context of their antifungal efficacy, mechanisms of action, and applications in veterinary medicine. By leveraging nanotechnology, innovative antifungal strategies may be developed to enhance therapeutic outcomes and address the limitations of traditional antifungal agents.