The use of nanobiomaterials in animal husbandry has significantly increased animal exposure. This raises concerns about nanosafety, genetic toxicity, and carcinogenicity. Although studies on the genetic toxicity of commonly used nanoparticles have yielded inconsistent results, many in vivo studies have demonstrated clear clastogenic, DNA-damaging, and mutagenic effects. These genotoxic effects are highly dependent on nanoparticle properties such as size, assembly, preparation methods, coatings, and dosage. These factors influence the interaction of nanoparticles with living cells or tissues, affecting their potential for genotoxicity and mutagenicity in various fields, including animal husbandry. This chapter explores the development and application of nanomaterials, particularly in safety and risk assessment in livestock management. As the use of nanomaterials proliferate, concerns about their toxicity and environmental impact have emerged in animal husbandry. The ability of nanoparticles to enter an animal's bloodstream through ingestion, inhalation, or skin contact and accumulate in organs raises critical questions about long-term safety. Moreover, the regulatory landscape for nanomaterials in animal husbandry is examined in India and globally. Guidelines from various agencies like EFSA, DBT, ICAR, and FSSAI provide a framework for ensuring the responsible use of nanomaterials. Despite these advancements, there is a necessary need for more robust regulations, risk assessments, and safety protocols to mitigate potential risks to both animals and the environment. The usage of nanomaterials raises ethical issues as well as legal and financial concerns. Researchers investigated various possible paths for future generations on the appropriate dosage of nanomaterials for sustainable benefits in livestock production and health.