Milk is the first diet of every mammal. It is a fundamental component of the human diet and a significant economic commodity globally. Ensuring its quality and safety is of paramount importance for public health, consumer satisfaction, and economic sustainability in the dairy sector. With the advent of nanotechnology, novel opportunities have emerged to enhance milk production, improve its nutritional profile, and ensure safety from farm to table. This chapter explores the application of nanomaterials in improving milk quality in dairy animals and dairy products, including mechanisms of action, types of nanomaterials used, benefits, potential risks, regulatory considerations, and future prospects.