E-healthcare continue to aid in the modernization of healthcare frameworks. WMSNs, given the openly accessible nature of the communication channel and the distribution of sensors in the external environment, are susceptible to a wide spectrum of possible security vulnerabilities. Authentication is one of the primary steps to maintain the protection of patient data and identity. This work presents an authentication framework utilizing Physical Unclonable Function (PUF). The utilization of session keys computed using the PUF will avoid the requirement of storing public keys in the device, hence enhancing the model’s reliability. Several methods are compared with the proposed one, proving its low communication overhead of only 1568 bits and 0.0068 ms as the computational overhead. Security validation is done by the Scyther tool validating its security and reliability.