Responding to the poor regulation of a single integrated energy system (IES) and the failure of traditional demand response (DR) to accommodate the interests of multiple parties. A Stackelberg Game optimization mechanism for a multi-integrated energy system (MIES) accounting for DR is proposed. The methodology is initiated with the construction of the MIES and the user-side model. The energy interaction mechanism of the MIES and the DR mechanism of the user side are then designed, and the respective objective functions and constraints are given. Subsequently, the Stackelberg Game model is constructed, with the MIES acting as the leader and users following in a subsequent position. The proposed model is then solved using the Tuna Swarm Optimization (TSO) algorithm and the Gurobi solver. Finally, the simulation example shows that the operating cost of the proposed MIES optimization mechanism is reduced by 16.49%, compared to the conventional model, indicating the effectiveness of the proposed model in this paper.