In anisotropic shale formation, a complete of elastic stiffness coefficients are required for estimation of anisotropic elastic properties and horizontal stresses. A series of ultrasonic velocity measurements and static triaxial tests are performed on plugs of deep Longmaxi shale for obtaining correlations between the dynamic and static elastic properties. Then the well log-based as well as laboratory measurements are used to predict depth-by-depth horizontal stresses. The results show that the static stiffness coefficients are smaller than their corresponding dynamic results but the static Biot’s coefficient is higher than dynamic value. And there are good linear regression correlations between above dynamic and static parameters. In the prediction of horizontal stresses, one hand the relative error significantly decreases after using static parameters, on the other hand the isotropic model underestimates the horizontal stresses compared with VTI model. The Biot’s coefficient has a significant impact on predicting horizontal stresses. The outcomes of this work can potentially improve predictions of elastic properties and horizontal stresses for deep Longmaxi formation.