This chapter examines the essential function of bioremediation science in tackling the substantial issue of environmental remediation, with specific emphasis on groundwater and soil contamination. It examines the scope and expenses related to groundwater contamination, the regulatory structure overseeing remediation initiatives, and the identification of principal organic and inorganic pollutants. The chapter underscores the constraints of current technologies and stresses the pressing necessity for progress in separation science to provide more efficient and economically viable solutions. Additionally, it offers an extensive summary of separation methods relevant to environmental remediation. The primary emphasis is on incorporating green energy technology, including solar and wind power, into remediation operations. The chapter examines the benefits of employing these renewable energy sources, such as sustainability, economic efficiency, and diminished environmental impact. It also recognizes the difficulties linked to these technologies, including intermittency and initial capital expenditures. The chapter highlights the promise of bioremediation, augmented by green energy, as a promising and environmentally friendly method for environmental rehabilitation. This chapter seeks to establish a fundamental comprehension of the essential function of separation science and green energy technologies in tackling the urgent environmental issue of pollution.