Silicon nanoparticles (SiNPs) have emerged as a promising tool in plant genetic engineering, offering innovative solutions for the delivery of genetic material, enhancement of transformation efficiency, and improvement of crop resilience. Their unique physicochemical properties—including high surface area, tunable porosity, biocompatibility, and ease of surface functionalization—make SiNPs highly effective nano-carriers for DNA, RNA, and genome-editing tools such as CRISPR/Cas systems. Recent advances demonstrate their potential in facilitating precise gene delivery, improving stress tolerance, and even enabling DNA-free genome editing in important crops. Despite these exciting developments, challenges remain, including optimization of delivery methods across diverse plant species, assessment of potential phytotoxicity, and evaluation of environmental and biosafety risks. This chapter provides a comprehensive overview of the properties, synthesis, and applications of SiNPs in plant biotechnology, highlighting key case studies, addressing potential risks, and offering perspectives on future research directions. Ultimately, SiNP-based technologies have the potential to revolutionize modern agriculture by contributing to sustainable crop improvement and food security.