Metallic Nanoparticles in Plant Genetic Engineering
摘要
Plant genetic engineering achieved a breakthrough with metallic nanoparticles (MNPs) because they enable innovative methods to move genetic material into plant cells. Plants require different traditional transformation methods Agrobacterium-mediated transformation and biolistic particle delivery which limit success due to species restriction together with reduced efficacy and risks of tissue destruction. MNPs establish themselves as novel delivery tools because they possess adjustable surface toppings and large surface-to-volume dimensions and cell wall penetration abilities without mechanical pressure. The DNA delivery properties of nucleic acids using nanoparticles have been investigated through studies of various types of MNPs including gold (Au), copper oxide (CuO), titanium dioxide (TiO2), and iron oxide (Fe3O4). The different processes through which MNPs enable gene delivery occur concurrently. Genetic material enters the plant cell cytoplasm and nucleus through two methods which include wall penetration via endocytosis and diffusion. This mechanism allows the laboratory to perform either temporary or permanent genetic alterations which helps advance plant biotechnological possibilities. Moreover, the transformative process of plant tissue using MNP-based methods decreases toxicity levels and improves genetic stability helping scientists attain higher control of delivery processes leading to predictable gene expression levels. Current research focuses on resolving important issues regarding nanoparticle phytotoxicity, dosage optimization, and environmental safety despite their advantages. This chapter demonstrates how metallic nanoparticles used with specific properties can bring major changes to plant genetic engineering platforms.